Bamboo is the fastest growing plant currently known on earth, a property that enables it to be the best alternative as a future source of wood fi ber. This study investigated the effect of site and culm height on the physical and chemical properties of Yushania alpina culms grown in Ethiopia. Matured Yushania alpina 3 to 5-year-old samples were harvested from Hagere-Selam and Rebu-Gebeya sites. The culms were subdivided into three equal lengths (bottom, middle, and top), and the variations in physical and chemical properties between the two sites and the culm heights of Yushania alpina were investigated. The results showed that the average values of MC, basic density, tangential and longitudinal shrinkage of Yushania alpina culms for Hagere-Selam and Rebu-Gebeya sites were (91.78 and 80.32 %), (0.65 and 0.63 g/cm3), (6.63 and 5.84 %) and (0.63 and 0.56 %), respectively. The average values of cellulose, lignin, extractive and ash contents in the culms for Hagere-Selam and Rebu-Gebeya sites were (52.84 and 50.71 %), (26.55, and 26.04 %), (8.41 and 8.02 %) and (1.95 and 2.17 %), respectively. The results revealed that the site affected the MC, basic density, cellulose, lignin, extractive, and ash contents of Yushania alpina culms but not the tangential and longitudinal shrinkage. The culm height of Yushania alpina affected MC, basic density, tangential shrinkage, longitudinal shrinkage, cellulose, lignin, extractive, and ash contents. In the case of both sites, the highest percentages of MC, tangential and longitudinal shrinkage, and ash content were observed at the base and lowest at the top of the culms. On the contrary, both sites observed thehighest magnitude of basic density, cellulose and extractive at the top and lowest at the base of the culms. The variations in physical and chemical properties at different sites and culm heights infl uence the utilization of Yushania alpina culms for industries and end products.